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tcyP [2018-09-13 18:26:00]
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tcyP [2018-09-13 18:26:00]

cystine and diaminopimelate transporter
locus
BSU09130
pI
9.47
mw
48.82 kDa
protein length
463 aa Sequence Blast
gene length
1389 bp Sequence Blast
function
cystine and diaminopimelate uptake
product
cystine and diaminopimelate transporter
essential
no
synonyms
yhcL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.6|Dicarboxylate/amino acid:cation symporter]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    986,986 → 988,377

    The protein

    Catalyzed reaction/ biological activity

  • cystine-proton symporter [Pubmed|15262924]
  • transport of diaminopimelate [pubmed|29995990]

    • Kinetic information

  • K(m) = 0.6 myM cystine [Pubmed|15262924]

    • Structure

  • [PDB|3KBC] (from ''Pyrococcus horikoshii'', 28% identity) [Pubmed|19924125]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]

    regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of sulfate or cysteine ([protein|search|CymR]) [Pubmed|16513748]
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    Biological materials

    Mutant

  • MGNA-A657 (yhcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/657 NBRP B. subtilis, Japan]
  • 1A949 ( ''tcyP''::''spec''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A949&Search=1A949 BGSC]
  • BKE09130 (Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA
  • BKK09130 (Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA

    • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

    • References

  • 15262924,12193636,16513748,16513748,18763711,19924125,29995990