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tcyP [2018-09-13 18:26:00]
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tcyP [2018-09-13 18:26:00]

cystine and diaminopimelate transporter
Locus
BSU09130
Isoelectric point
9.47
Molecular weight
48.82 kDa
Protein length
463 aa Sequence Blast
Gene length
1389 bp Sequence Blast
Function
cystine and diaminopimelate uptake
Product
cystine and diaminopimelate transporter
Essential
no
Synonyms
yhcL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → Dicarboxylate/amino acid:cation symporter
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    986,986 → 988,377

    The protein

    Catalyzed reaction/ biological activity

  • cystine-proton symporter PubMed
  • transport of diaminopimelate PubMed

    • Kinetic information

  • K(m) = 0.6 myM cystine PubMed

    • Structure

  • 3KBC (from Pyrococcus horikoshii, 28% identity) PubMed

    • Localization

  • membrane associated PubMed

    • Expression and Regulation

    Operons

    Genes
    tcyP

    Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon

    • Regulation

  • repressed in the presence of sulfate or cysteine (CymR) PubMed
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    Biological materials

    Mutant

  • MGNA-A657 (yhcL::erm), available at the NBRP B. subtilis, Japan
  • 1A949 ( tcyP::spec), PubMed, available at BGSC
  • BKE09130 (ΔtcyP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA
  • BKK09130 (ΔtcyP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA

    • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

    • References

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